6Z8H
Crystal structure of Variant Surface Glycoprotein VSG13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.717, 68.341, 156.903 |
| Unit cell angles | 90.00, 92.55, 90.00 |
Refinement procedure
| Resolution | 48.140 - 1.380 |
| R-factor | 0.2178 |
| Rwork | 0.218 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6z8g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.123 |
| Data reduction software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.140 | 1.428 |
| High resolution limit [Å] | 1.378 | 1.378 |
| Rpim | 0.034 | |
| Number of reflections | 155980 | 15141 |
| <I/σ(I)> | 8.07 | |
| Completeness [%] | 97.1 | 94.49 |
| Redundancy | 3 | |
| CC(1/2) | 0.995 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 1.8-2.0 M (NH4)2SO4, 100 mM Tris.Cl pH 8.5 |
