6Z7Z

Porcine insulin in complex with the analytical antibody OXI-005 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	109.670, 171.550, 83.170
Unit cell angles	90.00, 135.89, 90.00

Refinement procedure

Resolution	47.990 - 2.400
R-factor	0.212
Rwork	0.210
R-free	0.25880
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6z7y
RMSD bond length	0.008
RMSD bond angle	1.125
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3714)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.990	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.142	1.082
Rmeas	0.169	1.277
Rpim	0.090	0.672
Number of reflections	41390	13981
<I/σ(I)>	8.9	1.25
Completeness [%]	99.2	97.45
Redundancy	3.4	3.4
CC(1/2)	0.990	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M magnesium chloride, 20 % (w/v) PEG 3350