6Z7I
Crystal structure of CTX-M-15 E166Q mutant apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.72 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.587, 45.693, 117.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.590 - 0.980 |
| R-factor | 0.1273 |
| Rwork | 0.126 |
| R-free | 0.14420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6qw8 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.690 | 1.000 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rpim | 0.051 | 0.649 |
| Number of reflections | 138530 | 6818 |
| <I/σ(I)> | 7.6 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.9 | 12.9 |
| CC(1/2) | 0.994 | 0.487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | 0.1 M Tris pH 8.0, 2.0 M ammonium sulphate |
