6Z71
Structure of the MATE family multidrug resistance transporter Aq_128 from Aquifex aeolicus in the outward-facing state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.81517 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.300, 71.500, 104.300 |
| Unit cell angles | 90.00, 100.90, 90.00 |
Refinement procedure
| Resolution | 19.978 - 3.500 |
| R-factor | 0.3138 |
| Rwork | 0.312 |
| R-free | 0.34190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fv6 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.978 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.178 | 0.840 |
| Rmeas | 0.210 | 0.989 |
| Rpim | 0.110 | 0.516 |
| Number of reflections | 14759 | 1453 |
| <I/σ(I)> | 6.4 | 1.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.991 | 0.618 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6 | 295 | 0.1 M MES 0.1 M Magnesium chloride 0.1 M Sodium chloride 30% PEG 600 (v/v) |
