6Z6Y
Galectin-8 N-terminal domain in complex with a sulfatide mimicking a sphingolipid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979257 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.640, 40.066, 40.701 |
| Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
| Resolution | 37.610 - 1.340 |
| R-factor | 0.1643 |
| Rwork | 0.163 |
| R-free | 0.19380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gze |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.335 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Number of reflections | 39289 | 6233 |
| <I/σ(I)> | 21.2 | 1.5 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 6.4 | 6.2 |
| CC(1/2) | 1.000 | 0.823 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 298 | 0.1 M Na-HEPES, pH 7.6, 10% PEG 4000 and 20% Isopropanol |
