6Z6Y

Galectin-8 N-terminal domain in complex with a sulfatide mimicking a sphingolipid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979257
Spacegroup name	C 1 2 1
Unit cell lengths	110.640, 40.066, 40.701
Unit cell angles	90.00, 99.80, 90.00

Refinement procedure

Resolution	37.610 - 1.340
R-factor	0.1643
Rwork	0.163
R-free	0.19380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gze
RMSD bond length	0.009
RMSD bond angle	1.335
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.340	1.340
Number of reflections	39289	6233
<I/σ(I)>	21.2	1.5
Completeness [%]	99.6	98.3
Redundancy	6.4	6.2
CC(1/2)	1.000	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	298	0.1 M Na-HEPES, pH 7.6, 10% PEG 4000 and 20% Isopropanol