6Z6S
Crystal structure of Uba4-Urm1 from Chaetomium thermophilum
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 197.735, 197.735, 99.566 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.870 - 3.153 |
| Rwork | 0.210 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1292108208 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.525 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.870 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 5.447 | |
| Rmeas | 0.066 | 4.720 |
| Rpim | 1.910 | |
| Number of reflections | 13003 | 1279 |
| <I/σ(I)> | 18.85 | |
| Completeness [%] | 99.8 | |
| Redundancy | 9.9 | |
| CC(1/2) | 1.000 | 0.297 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Hepes pH 7.5 20% (w/v) PEG 3350 |
