6Z4W

FtsE structure from Streptococcus pneumoniae in complex with ADP (space group P 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97923
Spacegroup name	P 1
Unit cell lengths	33.387, 36.711, 41.106
Unit cell angles	105.27, 95.69, 99.69

Refinement procedure

Resolution	39.201 - 1.360
R-factor	0.1774
Rwork	0.176
R-free	0.19450
Structure solution method	AB INITIO PHASING
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.201	1.380
High resolution limit [Å]	1.360	1.360
Rmerge	0.074	0.932
Number of reflections	37746	1797
<I/σ(I)>	16.5
Completeness [%]	95.8
Redundancy	8.6
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.15 M NaF and 16% (w/v) PEG3350