6Z4V

Crystal structure of the neurotensin receptor 1 (NTSR1-H4bmx) in complex with NTS8-13

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000009
Spacegroup name	C 1 2 1
Unit cell lengths	69.223, 77.533, 111.858
Unit cell angles	90.00, 107.22, 90.00

Refinement procedure

Resolution	29.251 - 2.595
Rwork	0.256
R-free	0.27230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yvr
RMSD bond length	0.003
RMSD bond angle	1.216
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.251	2.802
High resolution limit [Å]	2.595	2.595
Rmerge	0.453	2.576
Rmeas	0.484	2.760
Rpim	0.165	0.970
Number of reflections	14177	709
<I/σ(I)>	4.3	1.5
Completeness [%]	88.9
Redundancy	8.5
CC(1/2)	0.921	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293.15	100 mM MES 350-450 mM ammonium tartrate 30% (v/v) PEG400 10 uM NTS8-13