6Z4V
Crystal structure of the neurotensin receptor 1 (NTSR1-H4bmx) in complex with NTS8-13
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000009 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.223, 77.533, 111.858 |
| Unit cell angles | 90.00, 107.22, 90.00 |
Refinement procedure
| Resolution | 29.251 - 2.595 |
| Rwork | 0.256 |
| R-free | 0.27230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6yvr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.216 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.251 | 2.802 |
| High resolution limit [Å] | 2.595 | 2.595 |
| Rmerge | 0.453 | 2.576 |
| Rmeas | 0.484 | 2.760 |
| Rpim | 0.165 | 0.970 |
| Number of reflections | 14177 | 709 |
| <I/σ(I)> | 4.3 | 1.5 |
| Completeness [%] | 88.9 | |
| Redundancy | 8.5 | |
| CC(1/2) | 0.921 | 0.458 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293.15 | 100 mM MES 350-450 mM ammonium tartrate 30% (v/v) PEG400 10 uM NTS8-13 |
