6Z2T
Three-dimensional structure of an influenza hemagglutinin LAH protein in its post-fusion conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 36.780, 36.780, 166.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.680 - 1.340 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gol |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.756 |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.520 | 1.420 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.050 | 0.583 |
| Number of reflections | 15916 | 2234 |
| <I/σ(I)> | 13.3 | 2.5 |
| Completeness [%] | 99.5 | 98.4 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 294 | 0.15 M H6NO4P and 40% MPD; pH 6.7; protein 10 mg/mL |
