6Z2B

Toprim domain of RNase M5 bound with two Mg2+ ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	C 1 2 1
Unit cell lengths	87.700, 30.320, 158.310
Unit cell angles	90.00, 100.89, 90.00

Refinement procedure

Resolution	43.675 - 2.138
R-factor	0.203
Rwork	0.199
R-free	0.28350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tg6
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.675	2.220
High resolution limit [Å]	2.138	2.140
Number of reflections	23254	2238
<I/σ(I)>	11.99
Completeness [%]	99.7
Redundancy	6.7
CC(1/2)	0.999	0.633

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M sodium acetate trihydrate pH 4.6, 30% (w/v) polyethylene glycol 4000