6Z1Z
Structure of the anti-CD9 nanobody 4C8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-16 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.901, 41.417, 56.853 |
| Unit cell angles | 90.00, 89.69, 90.00 |
Refinement procedure
| Resolution | 24.630 - 1.700 |
| R-factor | 0.2027 |
| Rwork | 0.201 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5imk |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.551 |
| Data reduction software | EVAL15 |
| Data scaling software | EVAL15 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.640 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.129 | 1.057 |
| Rpim | 0.083 | 0.677 |
| Number of reflections | 23719 | 2338 |
| <I/σ(I)> | 4.1 | 0.8 |
| Completeness [%] | 94.1 | 93.4 |
| Redundancy | 3.4 | 3.4 |
| CC(1/2) | 0.993 | 0.429 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 20% (w/v) PEG 8,000 The crystal was cryoprotected by soaking in reservoir solution supplemented with 30% (v/v) PEG 400 |
