6Z0F
Crystal structure of the membrane pseudokinase YukC/EssB from Bacillus subtilis T7SS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-10 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 149.015, 83.410, 106.445 |
Unit cell angles | 90.00, 108.16, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.420 | 2.858 |
High resolution limit [Å] | 2.553 | 2.553 |
Rmerge | 0.227 | 1.482 |
Rpim | 0.101 | 0.579 |
Number of reflections | 26268 | 1314 |
<I/σ(I)> | 4.7 | 1.2 |
Completeness [%] | 92.3 | 62.1 |
Redundancy | 6.1 | 7.5 |
CC(1/2) | 0.979 | 0.588 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 15 %w/v PEG 2K, 0.1 M MgCl2, 50 mM HEPES pH 7.5 |