6YZE
Zinc metalloprotease ProA from native source
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-10-18 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5419 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.633, 54.604, 134.519 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.900 - 2.180 |
R-factor | 0.208 |
Rwork | 0.205 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nqx |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18rc4) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.590 | 50.590 | 2.290 |
High resolution limit [Å] | 2.180 | 6.880 | 2.180 |
Rmerge | 0.138 | 0.048 | 0.528 |
Rmeas | 0.153 | 0.054 | 0.603 |
Rpim | 0.066 | 0.023 | 0.283 |
Total number of observations | 82983 | 2986 | 8129 |
Number of reflections | 16137 | 591 | 2106 |
<I/σ(I)> | 9.7 | 21.6 | 2.5 |
Completeness [%] | 98.5 | 98.4 | 91.4 |
Redundancy | 5.1 | 5.1 | 3.9 |
CC(1/2) | 0.993 | 0.998 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 16 mg/ml native ProA 1 M sodium acetate pH 4.5 and 1.2 M tri-sodium citrate |