6YYL
Crystal structure of S. pombe Mei2 RRM3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 41 |
| Unit cell lengths | 75.532, 75.532, 70.759 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.630 - 1.890 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.19400 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 31476 | 4900 |
| <I/σ(I)> | 1.42 | 1.42 |
| Completeness [%] | 98.9 | |
| Redundancy | 3.75 | |
| CC(1/2) | 0.990 | 0.273 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 278 | 1 M ammonium sulfate, 0.1 M Bis-Tris pH5.5; 1% PEG 3350 |
