6YXB
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148K variant (space group P21)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9737 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.101, 110.538, 56.941 |
Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
Resolution | 56.950 - 1.500 |
R-factor | 0.1845 |
Rwork | 0.184 |
R-free | 0.20290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6yx4 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.296 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 110.540 | 110.540 | 1.530 |
High resolution limit [Å] | 1.500 | 8.220 | 1.500 |
Rmerge | 0.071 | 0.038 | 0.484 |
Rmeas | 0.087 | 0.047 | 0.589 |
Rpim | 0.049 | 0.026 | 0.332 |
Total number of observations | 228792 | 1404 | 11064 |
Number of reflections | 76240 | 480 | 3706 |
<I/σ(I)> | 10.6 | 25.5 | 2.6 |
Completeness [%] | 98.9 | 96.3 | 98.1 |
Redundancy | 3 | 2.9 | 3 |
CC(1/2) | 0.997 | 0.998 | 0.676 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.5M Ammonium Sulfate, 0.1M tri-Sodium citrate, pH 6.5, 1M Lithium sulfate |