6YWW
MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 103 |
| Detector technology | PIXEL |
| Collection date | 2018-04-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 64.377, 48.986, 66.187 |
| Unit cell angles | 90.00, 90.53, 90.00 |
Refinement procedure
| Resolution | 66.180 - 2.102 |
| R-factor | 0.2225 |
| Rwork | 0.221 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c2i |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.990 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.180 | 2.350 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 6178 | 310 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 87.0 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.991 | 0.311 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM HEPES 7.5, 300 mM NH4Cl, 30% PEG 2000, 1% Dioxane and 1 mM CaCl2 |
