6YWQ
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148H variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9737 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 53.730, 110.353, 38.869 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.900 - 1.270 |
| R-factor | 0.1629 |
| Rwork | 0.161 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rhf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 53.730 | 53.730 | 1.290 |
| High resolution limit [Å] | 1.270 | 6.960 | 1.270 |
| Rmerge | 0.076 | 0.061 | 1.305 |
| Rmeas | 0.083 | 0.069 | 1.447 |
| Rpim | 0.033 | 0.030 | 0.617 |
| Total number of observations | 388559 | 2311 | 16602 |
| Number of reflections | 61883 | 456 | 3005 |
| <I/σ(I)> | 14.3 | 28.7 | 2.3 |
| Completeness [%] | 99.9 | 98.8 | 99.8 |
| Redundancy | 6.3 | 5.1 | 5.5 |
| CC(1/2) | 0.997 | 0.990 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 18% w/v Polyethylene glycol 8,000 |
