6YWP
Structure of apo-CutA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97600 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.960, 70.233, 145.337 |
| Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
| Resolution | 46.660 - 2.250 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.480 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 41372 | 6624 |
| <I/σ(I)> | 8.43 | 1.31 |
| Completeness [%] | 98.3 | 97.9 |
| Redundancy | 3.46 | |
| CC(1/2) | 0.996 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298.15 | 0.1 M HEPES-KOH pH 7.5, 0.2 M lithium sulfate monohydrate and 25% polyethylene glycol 3350 |
