6YWO

CutA in complex with A3 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979000
Spacegroup name	P 1
Unit cell lengths	56.920, 56.950, 149.890
Unit cell angles	79.86, 87.12, 75.83

Refinement procedure

Resolution	33.260 - 1.900
R-factor	0.1777
Rwork	0.176
R-free	0.21260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	XXXX
RMSD bond length	0.009
RMSD bond angle	0.945
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.260	2.010
High resolution limit [Å]	1.900	1.900
Number of reflections	136433	21579
<I/σ(I)>	8.49	1.2
Completeness [%]	96.1	94.3
Redundancy	3.6	3.6
CC(1/2)	0.990	0.590

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298.15	0.1 M Bis-Tris pH 6.5, 0.2 M lithium sulfate monohydrate and 25% polyethylene glycol 3350