6YWH
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148D variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9720 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 53.661, 110.471, 39.043 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.310 - 1.070 |
| R-factor | 0.1767 |
| Rwork | 0.176 |
| R-free | 0.18840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rhf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.442 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 55.240 | 55.240 | 1.090 |
| High resolution limit [Å] | 1.070 | 5.860 | 1.070 |
| Rmerge | 0.044 | 0.028 | 0.967 |
| Rmeas | 0.048 | 0.031 | 1.059 |
| Rpim | 0.019 | 0.012 | 0.425 |
| Total number of observations | 644391 | 4547 | 30027 |
| Number of reflections | 101277 | 747 | 4970 |
| <I/σ(I)> | 16.1 | 50.8 | 2 |
| Completeness [%] | 98.4 | 99.8 | 98.3 |
| Redundancy | 6.4 | 6.1 | 6 |
| CC(1/2) | 0.999 | 0.999 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M DL-Glutamic acid monohydrate, 0.1 M DL-Alanine, 0.1 M Glycine, 0.1 M DL-Lysine monohydrochloride, 0.1 M DL-Serine, 0.1M MES monohydrate, 0.1M Imidazole, pH 6.5, 20% v/v PEG 500 MME, 10% w/v PEG 20000 |
