6YWG
Structure of Chloroflexus aggregans flavin based fluorescent protein (CagFbFP) Q148N variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 295 |
Detector technology | PIXEL |
Collection date | 2018-06-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 53.718, 110.559, 38.985 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.280 - 1.450 |
R-factor | 0.1788 |
Rwork | 0.178 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rhf |
RMSD bond length | 0.005 |
RMSD bond angle | 1.306 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 110.560 | 110.560 | 1.480 |
High resolution limit [Å] | 1.450 | 7.810 | 1.450 |
Rmerge | 0.078 | 0.035 | 1.303 |
Rmeas | 0.081 | 0.037 | 1.355 |
Rpim | 0.022 | 0.010 | 0.367 |
Total number of observations | 536224 | 3924 | 27970 |
Number of reflections | 41453 | 335 | 2101 |
<I/σ(I)> | 18.5 | 53 | 2 |
Completeness [%] | 98.6 | 99.9 | 99.9 |
Redundancy | 12.9 | 11.7 | 13.3 |
CC(1/2) | 0.999 | 0.999 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.06M Magnesium chloride hexahydrate, 0.06M Calcium chloride dihydrate, 0.1M MES, 0.1M Imid, pH 6.5, 20% PEG500, 10% PEG20000 |