6YWD
De novo designed protein 4H_01 in complex with Mota antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 143.710, 143.710, 90.223 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.040 - 3.200 |
| R-factor | 0.2104 |
| Rwork | 0.206 |
| R-free | 0.24720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jlr |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.797 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.100 | 2.960 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 757244 | 117130 |
| <I/σ(I)> | 12.6 | 1.1 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 14.7 | 14.2 |
| CC(1/2) | 0.997 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M bicine pH 9.3, 22% v/v PEG Smear Broad |
