6YUG

Crystal structure of C. parvum GNA1 in complex with acetyl-CoA and glucose 6P.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	110
Detector technology	PIXEL
Collection date	2018-04-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	55.693, 70.089, 71.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.773 - 1.950
R-factor	0.2732
Rwork	0.272
R-free	0.31340
Structure solution method	AB INITIO PHASING
RMSD bond length	0.009
RMSD bond angle	1.353
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	Arcimboldo
Refinement software	PHENIX ((1.10.1-2155_743: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.773	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.113	0.920
Number of reflections	20192	2638
<I/σ(I)>	6.9	1.1
Completeness [%]	95.9
Redundancy	4.7
CC(1/2)	0.998	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	NH4Cl HEPES glycerolethoxilate