6YUB

Crystal structure of Uba4 from Chaetomium thermophilum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-27
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	64.750, 74.170, 103.660
Unit cell angles	90.00, 106.36, 90.00

Refinement procedure

Resolution	47.628 - 2.195
R-factor	0.2103
Rwork	0.208
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jw3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.630	2.274
High resolution limit [Å]	2.195	2.195
Rmeas	0.141	1.356
Number of reflections	48108	4560
<I/σ(I)>	10.33	1.31
Completeness [%]	99.4
Redundancy	6.6
CC(1/2)	0.997	0.689

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	50 mM HEPES pH 6.9 and 23.5 % (w/v) PEG 4000