6YU8

RNA Methyltransferase of Sudan Ebola Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.28242
Spacegroup name	P 62 2 2
Unit cell lengths	153.761, 153.761, 105.354
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	82.620 - 1.841
R-factor	0.1959
Rwork	0.195
R-free	0.21510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gkd
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	XDS (20190315)
Data scaling software	autoPROC (1.0.5)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.10.3 (29-NOV-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.622	1.873
High resolution limit [Å]	1.841	1.841
Number of reflections	63696	3136
<I/σ(I)>	13.7	2.3
Completeness [%]	100.0	100
Redundancy	40	41
CC(1/2)	0.996	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293.15	8% PEG 20000 Tris 0.1M NaCl 0,15M