6YU7

Crystal structure of MhsT in complex with L-tyrosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.96864
Spacegroup name	P 1 2 1
Unit cell lengths	44.100, 49.900, 110.300
Unit cell angles	90.00, 96.80, 90.00

Refinement procedure

Resolution	29.830 - 2.300
R-factor	0.224
Rwork	0.223
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4us3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.830	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	20849	1985
<I/σ(I)>	7.8
Completeness [%]	97.4
Redundancy	3.3
CC(1/2)	0.991	0.472

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	Crystallized using HiLiDe with DOPC as added lipid. Crystals were obtained in 14-24% PEG400, 0.3-0.5M NaCl, 0.1M Tris-HCl or HEPES-NaOH pH 7.0, 5% Trimethylamine N-oxide (TMANO), 5% or 10% glycerol.