6YU6

Crystal structure of MhsT in complex with L-leucine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-19
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	44.230, 215.560, 50.170
Unit cell angles	90.00, 90.05, 90.00

Refinement procedure

Resolution	45.484 - 2.350
R-factor	0.1919
Rwork	0.185
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4us3
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.500	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	38291	3843
<I/σ(I)>	8.1
Completeness [%]	98.5
Redundancy	3
CC(1/2)	0.996	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	Crystallized using HiLiDe with DOPC as added lipid. Crystals were obtained in 14-24% PEG400, 0.3-0.5M NaCl, 0.1M Tris-HCl or HEPES-NaOH pH 7.0, 5% Trimethylamine N-oxide (TMANO), 5% or 10% glycerol.