6YU6
Crystal structure of MhsT in complex with L-leucine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-19 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.230, 215.560, 50.170 |
Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
Resolution | 45.484 - 2.350 |
R-factor | 0.1919 |
Rwork | 0.185 |
R-free | 0.22220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4us3 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.500 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 38291 | 3843 |
<I/σ(I)> | 8.1 | |
Completeness [%] | 98.5 | |
Redundancy | 3 | |
CC(1/2) | 0.996 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | Crystallized using HiLiDe with DOPC as added lipid. Crystals were obtained in 14-24% PEG400, 0.3-0.5M NaCl, 0.1M Tris-HCl or HEPES-NaOH pH 7.0, 5% Trimethylamine N-oxide (TMANO), 5% or 10% glycerol. |