6YSH

Lamin A 1-70 coil1A dimer stabilized by C-terminal capping

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97631
Spacegroup name	I 41 2 2
Unit cell lengths	108.847, 108.847, 130.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.580 - 2.830
R-factor	0.23972
Rwork	0.237
R-free	0.28131
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yf5
RMSD bond length	0.018
RMSD bond angle	1.909
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.580	2.880
High resolution limit [Å]	2.830	2.830
Rmerge	0.201	2.510
Rmeas	0.205	2.558
Rpim	0.040	0.488
Number of reflections	9665	482
<I/σ(I)>	11.5	1.7
Completeness [%]	99.9	99
Redundancy	26.4	27.2
CC(1/2)	0.999	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.1M NaH2PO4 H2O,0.1M KH2PO4,2.0M NaCl,0.1M Mes H20 pH 6