6YSG
Magnesium chelatase H subunit (ChlH) from Synechocystis sp.PCC6803 to 2.54 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98674 |
Spacegroup name | H 3 |
Unit cell lengths | 321.119, 321.119, 104.966 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 83.780 - 2.540 |
R-factor | 0.2046 |
Rwork | 0.202 |
R-free | 0.25520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zhj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.532 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.1) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 83.780 | 83.780 | 2.740 |
High resolution limit [Å] | 2.540 | 6.720 | 2.540 |
Rmerge | 0.113 | 0.032 | 1.341 |
Rmeas | 0.120 | 0.034 | 1.431 |
Rpim | 0.041 | 0.012 | 0.495 |
Total number of observations | 1143007 | 62742 | 227759 |
Number of reflections | 133090 | 7181 | 27461 |
<I/σ(I)> | 12.1 | 38.5 | 1.7 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 8.6 | 8.7 | 8.3 |
CC(1/2) | 0.999 | 0.999 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 0.8-1.2M Sodium citrate pH7.0 and 2 mM dithiothreitol |