6YS4

Structure of the Homo sapiens SAS-6 coiled-coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979155
Spacegroup name	P 1 21 1
Unit cell lengths	40.004, 62.004, 153.748
Unit cell angles	90.00, 96.28, 90.00

Refinement procedure

Resolution	48.150 - 2.110
R-factor	0.242
Rwork	0.239
R-free	0.28800
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.130
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.460	2.170
High resolution limit [Å]	2.110	2.110
Rmerge	0.089	0.917
Rmeas	0.106	1.087
Number of reflections	37930	939
<I/σ(I)>	7.14	1.66
Completeness [%]	85.1	27.9
Redundancy	6.5	6.1
CC(1/2)	0.989	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	100 mM amino acids mixture, 0.1 M Gly-Gly / AMPD buffer, 50% v/v of precipitant mix (25% w/v PEG 4000 and 40% w/v 1,2,6-Hexanetriol)