6YRZ

Crystal structure of FAP et pH 8.5 after illumination at 150K

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	150
Detector technology	PIXEL
Collection date	2019-09-28
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	90.470, 104.560, 156.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.819 - 1.824
Rwork	0.158
R-free	0.18810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yru
RMSD bond length	0.012
RMSD bond angle	1.728
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.000	87.000	1.930
High resolution limit [Å]	1.820	5.440	1.820
Rmerge	0.086	0.036	1.038
Rmeas	0.089	0.038	1.078
Number of reflections	66267	2678	10478
<I/σ(I)>	18.38	59.83	2.09
Completeness [%]	99.7	99.7	98.2
Redundancy	13.518	12.671	13.489
CC(1/2)	0.999	0.999	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	281	PEG 4000 25-40%, Na citrate 100 mM, spermidine 10mM