6YRX

Low-dose crystal structure of FAP at room temperature

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	298
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9677
Spacegroup name	I 2 2 2
Unit cell lengths	95.650, 105.650, 158.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.181 - 1.870
Rwork	0.154
R-free	0.18000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yru
RMSD bond length	0.011
RMSD bond angle	1.698
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	88.000	88.000	2.000
High resolution limit [Å]	1.870	5.250	1.870
Rmerge	0.109	0.040	1.064
Rmeas	0.124	0.046	1.208
Number of reflections	65032	2988	11946
<I/σ(I)>	7.58	27.15	0.98
Completeness [%]	98.1	93.5	99.7
Redundancy	4.278	4.437	4.287
CC(1/2)	0.996	0.998	0.436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	PEG 4000, Na citrate, spermidine