6YRV

Crystal structure of FAP after illumination at 100K

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-10
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97372
Spacegroup name	I 2 2 2
Unit cell lengths	89.960, 103.860, 156.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	86.000 - 1.940
Rwork	0.165
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6yru
RMSD bond length	0.011
RMSD bond angle	1.697
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.500	86.000	1.990
High resolution limit [Å]	1.940	8.670	1.940
Rmerge	0.106	0.033	0.780
Rmeas	0.116	0.036	0.849
Number of reflections	54600	684	3961
<I/σ(I)>	11.62	31.65	2.23
Completeness [%]	100.0	99.7	100
Redundancy	6.585	5.804	6.525
CC(1/2)	0.998	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	281	PEG 4000 25-40%, Na citrate 100 mM, spermidine 10mM