6YRU

Crystal structure of FAP in the dark at 100K

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-10
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97372
Spacegroup name	I 2 2 2
Unit cell lengths	89.690, 103.570, 155.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.503 - 1.780
Rwork	0.172
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ncc
RMSD bond length	0.011
RMSD bond angle	1.661
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	86.000	86.000	1.900
High resolution limit [Å]	1.780	5.000	1.780
Rmerge	0.086	0.026	1.132
Rmeas	0.096	0.029	1.269
Number of reflections	69240	3293	12174
<I/σ(I)>	11.02	39.53	1.03
Completeness [%]	99.3	98.4	99.4
Redundancy	4.727	4.565	4.838
CC(1/2)	0.998	0.999	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	281	PEG 4000 25-40%, Na citrate 100 mM, spermidine 10mM