6YRL

Structure of the Chlamydomonas reinhardtii SAS-6 coiled-coil domain, C2 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	174.299, 39.739, 88.598
Unit cell angles	90.00, 108.91, 90.00

Refinement procedure

Resolution	43.482 - 2.343
R-factor	0.2505
Rwork	0.248
R-free	0.29690
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.681
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.816	2.380
High resolution limit [Å]	2.343	2.343
Rmerge	0.061	0.386
Rmeas	0.067	0.481
Rpim	0.027	0.280
Number of reflections	21732	526
<I/σ(I)>	17.5	2.1
Completeness [%]	88.1	44.2
Redundancy	5.5	2.8
CC(1/2)	0.999	0.895

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.06 M divalent ions mixture, 0.1 M imidazole / MES buffer 20% v/v ethylene glycol 10% w/v PEG 8000