6YRL
Structure of the Chlamydomonas reinhardtii SAS-6 coiled-coil domain, C2 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-31 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.299, 39.739, 88.598 |
| Unit cell angles | 90.00, 108.91, 90.00 |
Refinement procedure
| Resolution | 43.482 - 2.343 |
| R-factor | 0.2505 |
| Rwork | 0.248 |
| R-free | 0.29690 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.681 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.816 | 2.380 |
| High resolution limit [Å] | 2.343 | 2.343 |
| Rmerge | 0.061 | 0.386 |
| Rmeas | 0.067 | 0.481 |
| Rpim | 0.027 | 0.280 |
| Number of reflections | 21732 | 526 |
| <I/σ(I)> | 17.5 | 2.1 |
| Completeness [%] | 88.1 | 44.2 |
| Redundancy | 5.5 | 2.8 |
| CC(1/2) | 0.999 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.06 M divalent ions mixture, 0.1 M imidazole / MES buffer 20% v/v ethylene glycol 10% w/v PEG 8000 |
