6YQY
Crystal structure of sTIM11noCys, a de novo designed TIM barrel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-21 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 50.452, 50.452, 132.412 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.146 - 1.876 |
| R-factor | 0.2226 |
| Rwork | 0.220 |
| R-free | 0.27010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bvl |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.15_3459) |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.150 | 1.940 |
| High resolution limit [Å] | 1.876 | 1.876 |
| Rmerge | 0.061 | 1.281 |
| Number of reflections | 14703 | 1410 |
| <I/σ(I)> | 27 | 1.9 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 23.3 | 23.2 |
| CC(1/2) | 1.000 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298.15 | 0.2 M Ammonium sulfate, 0.1 M Trisodium citrate pH: 5.6, 25% w/v PEG 4000. |
