6YQG

Crystal structure of native Phycocyanin in spacegroup P63 at 1.45 Angstroms.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033200
Spacegroup name	P 63
Unit cell lengths	108.208, 108.208, 66.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.860 - 1.450
R-factor	0.1787
Rwork	0.178
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ypq
RMSD bond length	0.008
RMSD bond angle	1.248
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.860	1.540
High resolution limit [Å]	1.450	1.450
Rmeas	0.096
Number of reflections	153519	24621
<I/σ(I)>	9.95
Completeness [%]	99.8
Redundancy	5.72
CC(1/2)	0.999	0.246

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0,8% Anesthetic alkaloids mix, 0,1M Buffer System2 ,50% Precipitant mix2