6YPQ

Crystal structure of native Phycocyanin from T. elongatus in spacegroup R32 at 1.29 Angstroms

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.033200
Spacegroup name	H 3 2
Unit cell lengths	187.169, 187.169, 59.927
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.790 - 1.290
R-factor	0.1529
Rwork	0.153
R-free	0.17010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jbo
RMSD bond length	0.008
RMSD bond angle	1.203
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.190	1.310
High resolution limit [Å]	1.290	1.290
Rmerge	0.035	0.409
Rmeas	0.037	0.453
Rpim	0.012	0.190
Number of reflections	99233	4327
<I/σ(I)>	30.3	3.8
Completeness [%]	99.1	87.9
Redundancy	9
CC(1/2)	0.999	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Morpheus: 0,1M Amino acids, 0,1M Buffer System 1, 30%v/v P500MME_P20K