6YP4

Putative adenylyl cyclase HpAC1 from Hippeastrum reveals a dominant triphophatase activity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9117
Spacegroup name	P 41 21 2
Unit cell lengths	59.808, 59.808, 146.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.388 - 1.945
R-factor	0.198401496391
Rwork	0.195
R-free	0.25228
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v85
RMSD bond length	0.007
RMSD bond angle	0.869
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.390	1.955
High resolution limit [Å]	1.945	1.945
Rmeas	0.111	0.696
Number of reflections	20207	3116
<I/σ(I)>	9.8
Completeness [%]	99.0	96
Redundancy	5.9	4.8
CC(1/2)	0.997	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200 mM sodium thiocyanate 20% PEG 3350