Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-04 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 92.310, 92.310, 100.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.198 - 1.200 |
| Rwork | 0.117 |
| R-free | 0.13530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a39 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.320 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.198 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmeas | 0.053 | 0.483 |
| Number of reflections | 135228 | 9151 |
| <I/σ(I)> | 26.7 | 3.2 |
| Completeness [%] | 99.8 | 96.3 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 12 mg/mL protein in 20 mM pH 8 Tris-HCl mixed 2:1 with 0.15 M sodium citrate, 0.8 M ammonium sulfate, 1 M lithium sulfate |
