6YOS

Binary complex of 14-3-3 zeta with Glucocorticoid Receptor (GR) pT524 pS617 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976251
Spacegroup name	P 43 21 2
Unit cell lengths	60.206, 60.206, 284.213
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.900 - 2.750
R-factor	0.24
Rwork	0.239
R-free	0.26100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o02
RMSD bond length	0.018
RMSD bond angle	1.460
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.900	58.900	2.900
High resolution limit [Å]	2.750	8.700	2.750
Rmerge	0.109	0.038	1.606
Rmeas	0.116	0.041
Rpim	0.036	0.013	0.607
Number of reflections	14321	570	1909
<I/σ(I)>	12.4
Completeness [%]	98.3	99.7	94
Redundancy	9.4	8.7	6.9
CC(1/2)	0.998	0.999	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.8	293.15	0.35 M MgCl2, 24.4% PEG 3350, 0. 1 M Bis Tris pH 5.5 supplemented with 10% of an additive buffer containing 0.06 M CHAPS, 0.06 M HEPES, 0.06 M Tris, 0.25% w/v Hexamminecobalt(III) chloride and 0.02 M HEPES sodium pH 6.8.