6YOE
Structure of Lysozyme from SiN IMISX setup collected by still serial crystallography on crystals prelocated by 2D X-ray phase-contrast imaging
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.500, 78.500, 40.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.510 - 1.850 |
| R-factor | 0.2644 |
| Rwork | 0.263 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d5f |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.445 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.510 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmeas | 0.260 | 1.200 |
| Number of reflections | 11247 | 1101 |
| <I/σ(I)> | 2.3 | 0.85 |
| Completeness [%] | 100.0 | 96.4 |
| Redundancy | 11.29 | 5.6 |
| CC(1/2) | 0.990 | 0.220 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 4.5 | 293 | 0.5-1 M NaBr, 50-100 mM CH3COONa, pH 4.5, and 15-30 %(v/v) PEG400 |
