6YO8
Binary complex of 14-3-3 zeta with Glucocorticoid Receptor (GR) pT524 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9159 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 158.651, 99.880, 84.812 |
Unit cell angles | 90.00, 93.73, 90.00 |
Refinement procedure
Resolution | 60.850 - 2.090 |
R-factor | 0.225 |
Rwork | 0.224 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o02 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.2) |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.474 | 2.122 |
High resolution limit [Å] | 2.086 | 2.086 |
Rmerge | 0.061 | |
Rmeas | 0.073 | |
Rpim | 0.039 | |
Number of reflections | 77152 | 3821 |
<I/σ(I)> | 9 | |
Completeness [%] | 98.3 | 97.8 |
Redundancy | 3.4 | 3.4 |
CC(1/2) | 0.997 | 0.296 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8.3 | 277.15 | 1.29 M MgCl2, 22.5% PEG 3350, 0.1 M Tris pH 8.3, supplemented with 10% of an additive buffer containing 40% v/v of 2,5-Hexanediol |