6YO1
Crystal structure of ribonuclease A solved by vanadium SAD phasing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 60 |
| Detector technology | PIXEL |
| Collection date | 2019-08-01 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 2.26 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.510, 32.660, 72.400 |
| Unit cell angles | 90.00, 90.83, 90.00 |
Refinement procedure
| Resolution | 41.560 - 1.900 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.21290 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.560 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.088 | |
| Number of reflections | 18916 | 1194 |
| <I/σ(I)> | 20.6 | |
| Completeness [%] | 100.0 | 99.6 |
| Redundancy | 23.6 | 18.3 |
| CC(1/2) | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 20 mM sodium citrate pH 5.5, 28 % PEG 4000 with 20 mM uridine/ortho-vanadate |
