6YND

GAPDH purified from the supernatant of HEK293F cells: crystal form 1 of 4.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-22
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97622
Spacegroup name	P 1 21 1
Unit cell lengths	81.885, 124.455, 141.986
Unit cell angles	90.00, 99.39, 90.00

Refinement procedure

Resolution	140.090 - 1.525
R-factor	0.1829
Rwork	0.182
R-free	0.19950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u8f
RMSD bond length	0.008
RMSD bond angle	1.020
Data reduction software	PROCOR
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3 (19-MAR-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	140.090	1.710
High resolution limit [Å]	1.520	1.520
Rmerge	0.080	1.071
Rmeas	0.090	1.161
Rpim	0.030	0.444
Number of reflections	295271	14763
<I/σ(I)>	11.7	1.7
Completeness [%]	69.4	12.2
Redundancy	6.9	6.8
CC(1/2)	0.997	0.608

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1M MORPHEUS Amino acids solution (DL-Glutamic acid; DL-Alanine; Glycine; DL-Lysine; DL-Serine), 0.1M MORPHEUS Buffer System 3 (Tris (base); BICINE), 30% v/v MORPHEUS Precipitant Mix 1 (40% v/v PEG 500* MME; 20 % w/v PEG 20000)