6YMT
RNASE 3/1 version1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 106.845, 115.676, 284.742 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.190 - 1.580 |
| R-factor | 0.1797 |
| Rwork | 0.178 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e21 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.063 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 142.371 | 1.636 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 239337 | 23748 |
| <I/σ(I)> | 13.04 | 1.83 |
| Completeness [%] | 99.9 | 99.95 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.998 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | sodium acetate 100 mM pH 4.0 , NaCl 1.5 M |
