6YMO

Binary complex of 14-3-3 zeta with Glucocorticoid Receptor (GR) pS617 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976251
Spacegroup name	P 21 21 21
Unit cell lengths	72.285, 104.351, 112.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.330 - 2.020
R-factor	0.213
Rwork	0.212
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o02
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.330	47.330	2.070
High resolution limit [Å]	2.020	9.020	2.020
Rmerge	0.082	0.031
Rmeas	0.089	0.034
Total number of observations	366684
Number of reflections	56557	714	3957
<I/σ(I)>	12.07		0.53
Completeness [%]	99.6	98.1	95.8
Redundancy	6.483	5.419	6.344
CC(1/2)	0.999	0.999	0.226

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.8	277.15	2.04 M ammonium sulfate, 0.2 M sodium citrate supplemented with 10% of an additive buffer containing 0.33% w/v 3-aminobenzoic acid, 0.33% w/v 3-aminosalicylic acid, 0.33% w/v salicylic acid and 0.02 M HEPES sodium pH 6.8