6YMM
Crystal structure of the SAM-SAH riboswitch with SAM from space group P312
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-21 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9198 |
| Spacegroup name | P 3 1 2 |
| Unit cell lengths | 88.170, 88.170, 76.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.180 - 2.200 |
| R-factor | 0.1939 |
| Rwork | 0.192 |
| R-free | 0.22110 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.639 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.080 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.081 | 1.500 |
| Rpim | 0.022 | 0.520 |
| Number of reflections | 32912 | 801 |
| <I/σ(I)> | 14.2 | 1.4 |
| Completeness [%] | 99.6 | 95.7 |
| Redundancy | 14.2 | 9.3 |
| CC(1/2) | 0.970 | 0.696 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.01 M Magnesium Sulfate, 0.05 M Sodium Cacodylate pH 6.0, 1.8 M Lithium Sulfate monohydrate |
