6YM1

Mycobacterium tuberculosis FtsZ in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	86
Detector technology	PIXEL
Collection date	2019-12-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 65
Unit cell lengths	88.542, 88.542, 178.116
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	76.680 - 1.700
R-factor	0.1769
Rwork	0.175
R-free	0.20770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rq7
RMSD bond length	0.012
RMSD bond angle	1.230
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.680	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.093
Number of reflections	86575	12573
<I/σ(I)>	9.5
Completeness [%]	100.0
Redundancy	8
CC(1/2)	0.998	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	291.15	27.5% PEG4000, 0.4 M ammonium acetate and 0.1 M Na citrate, soaked with 5 mM GDP for 2 hr