6YLO

mTurquoise2 - Directionality of Optical Properties of Fluorescent Proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-21
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.54187
Spacegroup name	C 2 2 21
Unit cell lengths	60.820, 90.550, 117.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.920 - 1.700
R-factor	0.18
Rwork	0.179
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ztf
RMSD bond length	0.016
RMSD bond angle	1.644
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.920	58.920	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.058	0.024	0.777
Rmeas	0.063	0.025	1.013
Total number of observations	217990
Number of reflections	34680	453	1589
<I/σ(I)>	17.43	72.31	0.8
Completeness [%]	95.9	98.5	60.4
Redundancy	6.286	12.302	1.566
CC(1/2)	0.999	1.000	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	PEG 3000, Tri-sodium citrate